Experimental expertise
Molecular and cell biology
Single-cell RNA sequencing (10X genomics)
Next-generation sequencing (Illumina HiSeq-4000, 2500)
Microarray (Affymetrix)
NanoString gene expression assays for miRNAs
ELISpot, Western Blotting, ELISA
Transfection
Droplet digital PCR, Quantitative (qRT-PCR), semi-quantitative PCR for gene expression and functional analysis
Isolation of DNA and RNA (From Blood/Tissue/Saliva), cDNA preparation, PCR-SSP, Multiplex-PCR and PCR-based RFLP, Amplified refractory mutational system (ARMS)-PCR, SSCP (Single-Stranded Conformation Polymorphism), SnapShot-PCR, SDS-PAGE
Mixed Lymphocyte Reaction, Primary, and secondary cell culture
Immunohistochemistry, Immunocytochemistry
Histocompatibility
Serological typing by complement-dependent lymphocytotoxicity
Panel reactive antibody test using enzyme-linked immunosorbent assay (ELISA)
Detection of donor-specific antibodies (DSA)
Molecular HLA typing: Sequence-specific oligonucleotide probe typing (SSOP), Sequence-specific primer-based typing (SSP), DNA sequencing
Experimental animal handling
Extensive expertise in handling multiple animal models including mice, hamsters, guinea pigs, and rabbits while involved in COVID-19 and Herpes vaccine research
Highly experienced to work in ABSL3 and ABSL2 laboratories
Bioinformatics and Bio-Statistics
R and Python Programming Language
Computer Aided Drug Designing (CADD): Structure based drug design (SBDD) and ligand based drug design (LBDD)
Molecular Dynamics Simulation
Proficient in analyzing DNA and RNA sequencing data
Proficient in sequence analysis and alignment tools: Sequence analysis software (ABI), DNA Star, Gene Scan, BioEdit, DnaSP etc.
Copy Number Variation (CNV) data analysis: Chromosome Analysis Suite
Variant analysis: Alamut visual
Tools for predicting impacts of variations: Meta-SNP, MutationTaster2, MutPred, Predict-SNP, PROVEAN, DDIG-in, HMMvar, SIFT Indel, DeepSEA, sTRAP, PolyPhen-HCM, POSE etc.
Protein modeling: Modeller9.11, JMol, Clustal-W etc
Pathway analysis: GSEA, GOEAST, Gene Ontology, KEGG, WebStalt etc.
Phylogenetic analysis: MEGA, Phylip, NETWORK, MAFTT
Statistical packages: SPSS, Arleqin, Popgene, Haploview, Emhapfrequest, Minitab, Instant Graph pad, PRISM, Multifactor Dimensionality Reduction (MDR), Random Forests (RF), Classification and Regression Tree (CART) and Grade of Membership (GoM) analysis etc.